Immunoassays are methods that have become quite popular for detecting proteins. Those proteins can be an antibody and cytokines. These immunoassays are made to use them in the laboratory and field.
The way that proteins and immunoassays have been used together is to be able to get a more secure and on-point diagnostic for many diseases. Those diseases being Alzheimer’s, hepatitis, cancer, many autoimmune disorders.
There are two types of immunoassays. The first being the homogeneous and the second heterogeneous. Out of the two, the more common one used is heterogeneous. The most known type of heterogeneous assay is ELISA.
When it comes to proteins, we often think that they are only something we need to consume in our food. But, in fact, proteins are much more than that. Those are microscopic molecules. They can be found in a cell in our bodies.
They are responsible for many vital functions in our bodies. It all depends on the shape of the protein, its formation, and ways of usage. If you want to know more, follow the link https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741188/.
So it is very important to understand the stability of proteins. This is something that is important for the pharmaceutical industry, the food industry. So, if you know the whole process of stabilizing a protein, then you entirely use it for academic researches.
You have to understand that they don’t stay the same all the time. When they are in a cell, they keep getting degraded.
Keeping the proteins stabilized is crucial for the functions of the proteins. When they are stabilized, they are in an active state. In contrast, when some unwanted conditions are messing with the protein, it becomes inactive.
To be able to find new ways to stabilize any protein is going to be an advancement in the biotechnological and pharmaceutical industry.
One of the most essential parts of the protein is thermodynamic stability. This part is crucial when it comes to the structure, functions of the protein. The question that has been going around is how do thermophilic proteins live and exist in places where the temperatures got so high to reach the point of water boiling.
How can they survive in such high temperatures, and more importantly, how can they adapt to it? The way they do it is by mutating. With each mutation, they become more stable. By comparing proteins such as thermophile and mesophile, scientists have been able to figure out how one protein acts.
Few methods can help stabilize these proteins. Molecular dynamics is one of them. How this is done is usually there are two of them which have been put in few different temperatures. This is done to be able to see how these proteins react in various temperatures and how they bind. Check this page out if you want to know more about this.
Another method is via a unique stability characterization tool called the NanoTemper Prometheus system. Hailed as the new gold standard for research and development, Prometheus is able to achieve an unparalleled protein stability profile by utilizing simultaneous criteria such as precise measurements of aggregation, particle sizing, chemical denaturing, and thermal unfolding. More importantly, researchers are able to run several samples at a time, minimizing user error and maximizing accuracy.
How to determine the stability of a protein?
As we saw before, it is very important for scientists and biopharmaceuticals to be able to use proteins to prevent any diseases or to control them. There are a lot of new technologies that have found a way to store these proteins for a long time since they can be very unstable.
One of the most used techniques is the thermal unfolding method. With this method, we can discover at which temperature the protein has substantial changes. These changes can be used to provide a longer life for the protein.
No matter what kind of method is used, the end result is continuously collected by the DSC. DSC is the Differential scanning calorimetry. With DSC, many manufacturers actually see if their technology is approved. With DSC, protein stability assays can be approved.
A lot goes into experimenting and researching with these assays. It is crucial to understand the functionality and behavior of these proteins to be able to get full use of them. They can be the crucial part of discovering diseases and finding ways to actually cure them.
In the last decade or so, it has become a trend to develop and outsource the pharmaceutical products from testing out protein stability. Since this has become one of the leading ways to discover new and already existing diseases, many manufacturers have joined this industry.
It has had significant growth over the last hundred years. As new technologies thrive, these manufacturers just try to incorporate them in their experiments and researches to find new innovations. These innovations can create new ways of using proteins to our advantage.
We may be able to find out about all kinds of diseases in a faster and more efficient way. That’s why it is crucial to understand the way a protein works and handles different conditions. New mutations from it can be new ways to manage and cure diseases.